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What formula is used to calculate the Severity Scoring for my PCR Assay?
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Solved 9. The melting temperature for PCR primers is | Chegg.com
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Solved 8. Calculate the Tm of our primers (use the | Chegg.com
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Oligonucleotide Melting Temperature
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SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly on length and composition of the primer(s). An annealing temperature 5°C
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SOLVED: Use the Wallace Rule equation to calculate the Tm of each of the primers you designed. Tm = 2(A+T) + 4(G+C) Left primer: 5' CCTGGACACTGTAC 3' Right primer: 5' AATGTATGAACCTG 3
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