Sequences and melting temperature (Tm) of PCR primers used in this study. | Download Table
BISR Primer
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What is Annealing Temperature in PCR? How to Calculate and Set It? – Genetic Education
Understanding melting temperature (Tm)
Primer Design
SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule" This equation is: Tm = 2(A+T) + 4(G+C) Where A = # of
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